Comparison of small intestine fatty acid composition of mice fed with different types of diet

The aim of the study was to determinate the changes in intestinal fatty acid composition of mice fed with high fat or high fructose diets. In the beginning of the study, the wash out period; C57BL / 6 type male mice (n = 40, 8 weeks old) were fed with ad libitum standard diet for 2 weeks. Then, the intervention period (15 weeks); the mice which were randomly placed into 4 groups fed with ad libitum different diets; standard diet (SD) (as the control), high fructose diet (HF), high monounsaturated fatty acids diet (HMUFA), high saturated fatty acids diet (HSFA). At the end of the intervention, the fatty acids methyl esters were derived from intestine samples and measured by gas chromatography method. Highest food intake was determined in the HF (p <0.05). Higher energy intake and higher body weight gain was detected in intervention dietary group than the control (p <0.05). The highest total SFA and the lowest total MUFA percent was contained in HSFA group (p <0.05). There was no difference between the groups in total PUFA percent (p> 0.05). The highest caproic acid (C6: 0) percent was detected in the HF and different from HMUFA group (p <0.05). The highest Myristic acid (C14: 0) was observed in the HSFA (p <0.05). The lowest oleic acid (C18: 1) was detected in the HSFA and different from the SD and HF (p <0.05). Linoleic acid (C18: 2) and dihomo gamma linolenic acid (C20: 3) were found significantly different between SD group and HSFA group (p <0.05). The lowest docosahexaenoic acid (DHA) (C22: 3) percent was observed in the HSFA (p <0.05). In conclusion, it has been found that diet types could cause differences in the fatty acid composition of small intestine. In addition, while decreasing the total MUFA ratio, high fat and high SFA consumption increased the total SFA ratio in the small intestines. Key words: Saturated fatty acids, monounsaturated fatty acids, fructose, small intestine