Farmasötik müstahzarlarda antihistaminin (H1) spektrofotometrik tayini

In this study, the measurement of promethazine hydrochloride and thiamin hydrochloride was done in its pure form by applying highly senstive and accurate approach. After reacting the drug with coupling agent 4-aminoacetonilid in the presence of N-bromoschnimid, a soluble green colour complex was obtained. The maximum absorption of final product was measured at 606 nm and found that, the concentration range between (2.0 – 22) mg only obey the Beer's law, with a correlation coefficient of 0.9965, molar absorptivity of 9530.136 L.mol-1cm, a Sandel significance value of 0.0336 mg/cm3, LOD= 0.2129 mg.ml-1 and a relative standard deviation of 0.28. The recovery rate observed was almost 100.45%. For thiamin hydrochloride, a LOD= 2.71 mg/ml, an absorption coefficient of 5.3751 * 10-4, a Sandy significance of 0.005970 µg.cm-2 and relative standard deviation of 0.5778% was measured by using Th + PTA + DBP and Th+PTA+ACPH electrode. A bipolar recoverability value of 95.02% and 99.68% was observed for thiamin hydrochloride. This approach would have advantages for use in quality control applications because it is fast and accurate, as well as this method can be used for the determination of promethazine hydrochloride and thiamin hydrochloride at low-cost compared to other methods