The therapeutic efficacy of the quercetin molecule in glioblastoma multiforme cancer stem cells

Title: The Therapeutic Efficacy of the Quercetin Molecule in Glioblastoma Multiforme Cancer Stem Cells Aim: Glioblastoma multiforme (GBM) is a highly aggressive brain tumor characterized by poor prognosis and resistance to current treatment options. This study aimed to examine the potential therapeutic effects of quercetin, a naturally occurring flavonoid, on GBM stem-like T98G cells by evaluating its influence on cell proliferation, migration, and apoptosis. Material and Method: T98G cells were exposed to 100 µM quercetin for 24 hours. Cell viability was measured using the MTT assay, while migration capacity was assessed with wound healing assay. Clonogenic survival was determined by colony formation assay. Immunohistochemical staining was performed to detect protein expression of p-AKT, p-mTOR, beclin-1, active caspase-3, and Ki-67. Statistical analyses were conducted via SPSS software. Results: Quercetin treatment resulted in a significant decrease in migration and colony formation. A marked reduction in Ki-67 expression and an increase in apoptotic activity were observed. Furthermore, suppression of signaling pathways related to cell survival and proliferation was noted. Conclusion: The findings demonstrate that quercetin holds therapeutic promise in the context of GBM stem-like cells. Through the inhibition of proliferation and migration alongside the promotion of apoptosis, quercetin may represent a valuable candidate as a supportive agent in GBM treatment strategies. Further research is needed to confirm its clinical potential. Keywords: Flavonoid, Glioblastoma Multiforme, Quercetin, T98G