Hepato-protective and therapeutic effect of ellagic acid on D-galactosamine-induced liver damage

Polyphenol-rich dietary foodstuffs, consumed as an integral part of vegetables, fruits, and beverages have attracted attention due to their antioxidant properties. Ellagic acid (EA), a polyphenolic compound widely distributed in fruits and nuts, has been reported to scavenge free radicals and protect cellular membranes. This study examined the protective mechanism of EA against D-galactosamine (D-GalN)-induced liver damage. A little is known about the influence of EA on D-GalN toxicity in vivo. D-GalN, a well-known hepatotoxicant, causes severe hepatocellular inflammatory damage and clinical features similar to those of human viral hepatitis in experimental animals. A little is known about the influence of EA on D-GalN toxicity in vivo. Thirty-five healthy male Wistar rats were equally divided into five groups. First and second groups were used as controls. The control groups received 0.5 ml of normal saline and 0.2% (0.5 ml of DMSO), respectively. The third group received D-GalN (700 mg/kg). Fourth and fifth groups were recieved D-GalN (700 mg/kg) plus EA (25 mg/kg) and EA (25 mg/kg) plus D-GalN (700 mg/kg), respectively. The rats were killed at the end of the treatment period. The level of ALT, AST, ALP, and LDH were determined in liver tissue. Administration of D-GalN increased the level of these liver enzymes when compared to the control group. The administration of EA to D-GalN-treated rats decreased the ALT, AST, ALP, and LDH levels in these samples. D-GalN caused marked damages in the histopathological status of liver tissues. These damages were ameliorated by EA administration. In conclusion, EA may be used in combination with D-GalN in chemotherapy to improve D-GalN-induced liver damages. Key words: Ellagic acid, D-Galactosamine, Hepatoprotective effect, Liver damage, antioxidant