A comparative study on detection of B. avium antibodies in turkeys by enzyme-linked immunosorbent assay, microagglutination and serum plate agglutination tests

7. SUMMARYThe aims of this study were to develop a serum plate agglutination test (LAT)antigen and microagglutination (MAT) test antigen for the serologic detection ofturkeys that have been exposed to B. avium from with respiratory disease symptom andto compare sensitivity of commercial enzyme-linked immunosorbent assay (ELISA)with these tests.All serum samples were collected from commercial turkey enterprises in İzmir,samples from 900 turkeys with symptoms of respiratory disease constituted the materialof this study. The seroprevalence was determined as 21.6 %, 13.8 %, 12.1 % for ELISA,LAT, and MAT tests, respectively. The results showed that the difference of ELISAwith LAT and MAT tests was statistically significant (P<0.001).Consequently, our study showed that the seroprevalence of B. avium antibodieswere found as high in commercial turkey enterprises in İzmir. Although ELISA wasdefined as the most sensitive test for screening of B. avium antibodies with this study,LAT and MAT?s could also be used for this purpose.This project was supported by the Scientific and Technical Research Councilof Turkey (TÜBİTAK Project Number: 106 O 268).KEY WORDS: B. avium, Enzyme-Linked Immunosorbent Assay, MicroagglutinationTest, Plate Agglutination Test