HPLC method development and validation for the simultaneous determination of flurbiprofen and chlorhexidine gluconate

Flurbiprofen (FBP) is a strong nonsteroidal antiinflammatory drug, which has analgesic, antipyretic and antiinflammatory effects. Chlorhexidine gluconate (CHG) is a potent antibacterial agent. Nowadays, FBP and CHG in combination are used in some commercial preparations. Thereby, there is a need for a method that analyzes these two drugs together. In this study, an analysis method was developed for simultaneous determination of FBP and CHG by using high performance liquid chromatography (HPLC) technique. During analyses, Agilent Poroshell 120 EC-C18 column was used as stationary phase. Phosphate buffer solution (100 mM, pH 2.5) and acetonitrile were used as mobile phase. The chromatography was performed by gradient elution at a flow rate of 0.5 ml/min. The column temperature was set at 30˚C and the injection volume was 20 μl. Analytes were detected at 248 nm with using a diode array detector (DAD). The developed method was validated according to United States Pharmacopoeia guideline. The method was found to be linear in the concentrations range between 1–25 ppm. Correlation coefficient value was calculated as 0.9999 for FBP and CHG. The method was found suitable in terms of specificity, linearity, accuracy, and precision. It was applied successfully for HPLC analysis of commercial samples that includes FBP and CHG.