Investigation of the effects of jasmonic acid and salicylic acid applications on rosmatrinic acid production in rosemary (Rosmarinus officinalis L.) in vitro conditions

Plants are the main sources of natural compounds used in the pharmaceutical industry. Rosmarinic acid (RA) is a natural antioxidant phenolic compound with antiviral, antibacterial, anti-inflammatory, antiallergic, and anticancer activities. In this thesis study, it was aimed to investigate the effects of salicylic acid and jasmonic acid, two important signaling molecules in plants, on the production of rosmarinic acid in Rosmarinus officinalis L. (rosemary), which is of great medical importance. In the first stage, different sterilization methods were applied and the appropriate sterilization method was determined. Then, callus was obtained from leaf explants of rosemary plants. The callus obtained were grown in subculture in different doses of salicylic acid and jasmonic acid medium. The callus induction frequency was measured and rosmarinic acid accumulation was analysed. When all elicitor concentrations were compared, the best callus induction frequency was 84% with 50 mM salicylic acid treatment. At the same time, the highest amount of RA was determined as 0.753 mg/g t.a. in 50 mM salicylic acid treatment. The data obtained showed that in vitro cultures using rosemary leaf explants with the addition of elicitors such as salicylic acid and jasmonic acid can be successfully used as an alternative method for RA production.