The prognostic significance of the proliferative index (ki-67), tumour supressor gene protein index (p53) and glucose transporter proteins (glut1 and glut3) expressions in squamous cell carcinoma cases of the larynx

Objectives: The aim of this experimental study was to investigate therelationships of proliferative index (Ki-67), tumor suppressor gene protein index (p53),and glucose transporter protein (GLUT1 and GLUT3) expressions with conventionalprognostic parameters in squamous cell carcinoma cases of the larynx, and their effectson the survival durations.Materials and Methods: 79 cases who diagnosed with conventional laryngealsquamous cell carcinoma and who have undergone laryngectomy participated in thestudy. A representative parafin section from each tumor was immunostained with theKi-67, p53, GLUT1, and GLUT3 antibodies using the streptavidin-biotin method. Inexamining the relationships between these immunohistochemical results and theconventional prognostic parameters, the Pearson Chi-Square (χ 2) or Fisher's Exact testwas used. Survival duration was analysed using the Kaplan-Meier method and log-ranktest.Results: There was a positive correlation between Ki-67 PI and tumour size(p=0,023). The overexpression of p53 was found to be significantly associated withsome of the well-prognostic factors; p53 expression was positively correlated withtumor differentiation (p=0,036) and negatively correlated with perineural invasion(p=0,031). The survival duration was found significantly prolonged in cases with53GLUT1 (p=0,002) and GLU3 (p=0,011) positive score. The effects of Ki-67 PI and p53expressions on survival duration were not significant. No significant relationshipsbetween other clinicopathological characteristics and immunohistochemical results weredetectedConclusion: The results of the present study indicated that Ki-67 PI and p53expressions were not significantly associated with survival durations. The positivity ofGLUT1 and GLU3 significantly prolonged patient survival duration. Some well-prognostic factors (well-differentiated tumours and cases with no perineural invasion)were found to be significantly associated with p53 overexpression.Key Words: Larynx, squamous cell carcinoma, Ki-67, p53, glucose transporterproteins, GLUT1, GLUT3, prognosis.54