The investigation of intestinal protozoa from diarrhea case in Malatya

G. SUMMARY The existence of protozoa in Malatya and its vicinity was investigated in diarrheaic stool samples submitted from clinics and polyclinics to department of Parasitology in Turgut Özal Medical Center. The applied differantial diagnostic methods were compared and assessed. The stool samples were examined by native-lugol, trichrome and kinyoun asid-fast stains. Age and sex of each patient was recorded. From a total of 500 patient with diarrhea ( age range 0-60 years), The number of males were 262 (52.4%) and the females were 238 (47.6%). Protozoa were detection in 82 out of 500 diarrheaic samples (16.4%). They were 31 (6.2%) G intestinalis, 14 (2.8%) E. histolytica, 11 (2.2%) E. coli, 10 (2%) T. intestinalis, 8 (1.6%) Cryptosporidium sp., 4 (1%) B. hominis, 2 (0.4%) E. nana, 1 (0.2%) E. hartmanni and 1 (0.2%) C. mesnili. In one patient, three protozoa (71 intestinalis + G. intestinalis + E. coli ) were seen Protozoa were seen in 62 (12.4%) samples and 63 (12.6%) by applying native-lugol and trichrome stains respectively. In both stains ( native-lugol and trichrome), the detection rates of protozoa were as fellows; 26 (5.2%), 31 (6.2) for G. intestinalis, 11(2.2%), 14 (2.8%) for E. histolytica, 9 (1.8%), 1 1 (2.2%) for E. coli, T. intestinalis was only detection by native-lugol 10 (2%), 3 (0.6%), 4 (0.8%) for B. hominis. 1 (0.2%),2 (0.4%) for E. nana, 1 (0.2%). 1 0.2%) for E. hartmanni and C. mesnili were only detection by native-lugol 1 (0.2%) Cryptosporidium sp. were found in 8 out of 500 patients (1.6%) by Kinyoun asid-fast. On four samples (0.8%), Cryptosporidium sp. was seen in mixed infection with G. intestinalis For our knowledge, this in the first intestinal parasitic study that deals with diarrheaic stool in Malatya. Therefore, we are unable to compare our results with other studies in Malatya. On coclusion trichrome stain must be done on stool samples in addition to amoebiosis was suspection in order to make correct diagnosis. 69