Determination of antifungal susceptibility and isolation of candida SPP. from mastitic cattle milk

Isolation, identification and determination of antifungal susceptibility of Candida spp. that cause mycotic mastitis in cattle in Tekirdağ province and its counties in Marmara Region were aimed in this research. 100 mastitic milk samples were used that were collected from farms of the region by performing California Mastitis Test (CMT). Primarily, samples were cultured to SDA and were incubated separately at 25-37 °C. From the colonies that grown in SDA in 2-3 days, in paste stiffness, 0.5-1 mm in diameter, white or cream colored, uniformly bounded and that have distinctive yeast scent were subjected to gram staining. Under gram staining evaluation, microorganisms that were predefined as Candida spp. were conveyed to SDA and pure cultures were obtained. Pure isolates were identified by API 20C AUX rapid identification kit. Antifungal susceptibility rates of identified Candida spp. were determined by using disc diffusion technique. In antifungal susceptibility tests; Ketoconazole, Fluconazole, Miconazole, Amphoteracine B, Nystatin and Flucytosine containing discs were used. Candida spp. were detected in the 25 of the 100 mastitic milk samples. According to results of API 20C AUX rapid identification kit, 8 C. krusei (32%), 5 C. albicans (20%), 3 each C. boidinii, C. famata and C.kefyr (12%), 2 C. spherica (8%) and 1 C. thermophilia (4%) were identified from the 25 isolated strains. In the evaluation of test results of isolated strains that performed as in respect to M-44 directive of NCCLS (Method for Antifungal Disc Diffusion Susceptibility Testing of Yeasts; Approved Guideline); all strains were found % 100 susceptible to Ketoconazole and 100% resistant to Fluconazole, Miconazole, Amphoteracine B and Flucytosine. Two of the C. albicans (40%) and 1 of the C. krusei (12.5%) isolates were susceptible Nystatin while all the other isolates were moderately resistant to that.