Molecular investigation of mycoplasma species in sheep lungs withpneumonia

Acute/subacute or chronic pneumonic Mycoplasma infections in sheep and goats cause significant economic losses due to high morbidity and sometimes high mortality. However, Mycoplasma agents are difficult to produce. Therefore, the true frequency of the disease is unknown. In this study, it is aimed to investigate Mycoplasma species from pneumonia lesions found macroscopically in the lungs of sheep and goats slaughtered in slaughterhouses. For this purpose, lesioned lung tissues will be taken from slaughterhouses in Şanlıurfa and Adıyaman region, from sheep and goats with pneumonia under aseptic conditions and will be delivered to the routine diagnostic laboratory of Harran University Veterinary Faculty Microbiology Department in a short time under cold chain conditions for necessary analysis. The lesioned lung tissues that cannot be reached will be frozen at -20 degrees without losing time and stored until the time of analysis. At least 100 lung tissues will be tested in the study. Direct DNA analysis will be made from the samples, and at the same time, DNA isolation will be carried out from these media by planting in the media for culture purposes. Using genus and species-specific primer pairs using DNA samples obtained later, probable species were identified as M. ovipneumoniae, M. mycoides subsp. capri, M. capricolum subsp. capripneumoniae, M. capricolum subsp. capricolum will be tested for M. agalactiae and M. arginine.