Cu(II) chelation and cytochrome-c adsorption to poly (2-hydroxyethylmethacrylate-co-methacrylamidohistidine) microbeads

11 ABSTRACT Master of Science Thesis Cu(H) CHELATION AND CYTOCHROME-c ADSORPTION TO POLY(2-HYDROXYETHYLMETHACRYLATE-co- METHACRYLAMİDOHİSTİDİNE) MICROBEADS Sibel EMİR DİLTEM İZ Anadolu University Graduate School of Natural and Applied Sciences Chemistry Program Supervisor: AssocProf. Rıdvan SAY 2002, 61 pages In this study, development of poly(2-hydroxyethylmethacrylate-co- methacrylamidohistidine) |p(HEMA-co-MAH)] metal-chelate affinity sorbents and its usefulness for cytochrome-c adsorption were researched. In the first step, p(HEMA-co-MAH) microbeads were prepared for cytochrome-c specific metal chelate affinity sorbent. The characterization of synthesized polymer was evaluated by elemental analysis, 'H-NMR and FTIR. Then, Cu(II) ions were loaded to p(HEMA-co-MAH) microbeads and the effect of pH and Cu(II) initiation concentration on loading of Cu(II) were investigated. In the third stage, the conditions like Cu(II) initiation concentration, cytochrome-c initiation concentration, buffer type, pH and ionic strength for the adsorption of cytochrome-c from aqueous solution were studied. Finally, desorption of adsorbed cytochrome-c on polymeric microbeads and reusebiliry of microbeads were investigated. Keywords: Cytochrome-c, Metal Chelate Affinity Chromatography, Protein-Metal Interaction, Proteins.