Preparation of the spme fibers by electropolymerization and its application in voltammetric and chromatographic determination of endocrine disruptor pesticides

The pesticides are used in agricultural production for food protection but, their residue in food alters the endocrine system. Boscalid is a broad spectrum fungicide for beans, berries and tomatoes. MRL values of Boscalid were established as 1 mg/kg for various types of food and therefore, sensitive and accurate analysis methods are required for BOS determination in food samples. In this thesis, it was aimed to develop electrochemical and chromatographic methods for Boscalid determination in tomato juice and puree samples. Electro-chemical studies include indirect determination of Boscalid in the presence of Co(II) ions by AdCSV in sub nanomolar levels. The catalytic character of the reduction process was established by mechanistic studies. Chromatographic studies include the use of a commercial (PDMS-DVB) and a lab made (PPy) solid phase microextraction (SPME) fibers. Experimental parameters such as pH, stirring rate and extraction time was optimized in tomato juice sample medium. The calibration graphs were drawn in ppb ranges. Overall results have demonstrated that Boscalid can be determined by both methods sensitively and selectively and the methods developed were applied to the real samples. Keywords: Boscalid, voltammetry, gas chromatography, solid phase micro extraction, tomato juice and puree