The use of Tyrphostin AG 556 in the prevention of ischemia reperfusion injury in unilateral testicular torsion in rats.

Testes torsion is an emergent condition that is particulary seen in neonatal and adolescence age groups and may lead to testicular atrophy. lt is one of the causative factors of infertilitiy. As well as degree and duration of torsion, the damage in torsion is very closely related to ischemia-reperfüsion period arising after detorsion. We may have some strong restrictions to change degree and duration of torsion, howewer it seems to be possible that we can change the steps after detorsion. Hence, all attempsts for improving testicular damage after torsion consentrate on period following detorsion. Her we studied tyrphostin AG 556 thought to inhibit formation of free oxygen radicals increasing in reperfüsion period and responsible for damage. İn this study, there were total 24 Sprague-Dawley rats in 4 groups. Surgical procedures were performed under ketamine anesthesia (80 mg/kg) given intraperitonealy. By rotating left testis 720º clockwase ,the torsion was achieved. Testes were left hours as torsioned, 4 hours for reperfusion. Group 1 was basal scale group and used for standartdization of biochemical data and histological appearense. They underwent only orchiectomy. Group 2 was operation group and torsion and detorsion were performed. Group 3, given tyrphostin AG 556 intraperitoneal with an amonut of 2 mg/kg 5 minutes before detorsion, also had the same surgical procedures was grup 2. Grup 4, plasebo-DMSO group, had intraperitoneal DMSO injection 5 minutes before detorsion. In all groups, orchiectomy was performed. We studied the effect of tyrphostin AG 556 in limiting testicular damage after detorsion by determining tissue MDA level, an index for lipid peroxidation and examining specimens histologically by light microscopy. MDA levels were fonud to be increased for all groups, but group 1. Among other 3 groups there was no staticaly significant difference. Histopathologicaly, group 3 had no statical difference with group 1. Still other groups were different. We showed that 2 hour 720º testes torsion caused testicular damage. Tyrphostin AG 556 couldn't decrease MDA level. Howewer lt led to some protection histologicaly. To conclude, we had testicular damage with 2 hour 720° torsion, and with tyrphostin AG 556 we showed histological protection observed by light microscopy. Yet it caused no decrease in MDA level, therefore, we suggest that to evaluate the positive histological etfect of tyrphostin AG 556, further experiments including differrent dose group and duration of torsion and reperfusion, should be done.