Characterization of SPBC409.08 and SPAC9.02c genes in Schizosaccharomyces pombe

Polyamines are molecules known to have important roles in control of the cell cycle and in fighting against stress in the cell. Of polyamines spermine and spermidine are known to bind DNA and change transciptome when they are added to the cell extrinsically. In the cell, the mechanism and modification of polyamine is regulated by cooperation of many proteins; for example polyamine carrier proteins and polyamine acetyltransferases. In this study, we aimed to discover the potential roles of SPBC409.08 and SPAC9.02c genes in the cell cycle and stress response in Schizosaccharomyces pombe. These genes are thought to be spermine family transporters and polyamine N- acetyltransferases due to similarity of sequences and their experimental characterization have not been carried out before. In the experiments wild type h- and h+ S. pombe were used. Deletion mutants of SPBC409.08 and SPAC9.02c genes were generated by Bahler method (Bahler, et al., 1998). Deletion was verified by colony PCR method. In next step, the cell cycle analysis and analysis of sensibility to stress were carried out on those mutants. The growth chart, cell size analysis, morphologic analysis, sporulation tests, mitosis and meiosis were observed to understand roles of the genes in the cell cycle. Moreover, the cells were exposed to different stress conditions and the potential roles in response to stress were examined. Osmotic stress and DNA damage were some of stress conditions carried out to the cells. The first step in characterized of genes is to achieve deletion of those genes in S. pombe. This is the first report about characterization of SPBC409.08 and SPAC9.02c genes in S. pombe.