Purification and biological activities of phecoerrithir from cyanobacteria

Cyanobacteria are prokaryotic creatures and are one of the photosynthetic organisms that can live in different habitats and have various chemical compounds. Phycobiliproteins are water-soluble, light energy absorbing proteins with naturally high fluorescent properties produced from cyanobacteria and eukaryotic algae. Phycoerythrin; red colored phycobiliproteins and phycocyanin are named as blue colored phycobiliproteins. In this study, phycoerythrin was extracted from a cyanobacteria species containing high amounts of phycoerythrin, which was previously isolated and added to our culture collection, and it was partially purified by chromatographic techniques. The species known to show 99.23% similarity with Leptolyngbya compacta as a result of 16S rRNA ITS sequence analysis of dark red-brown filamentous cyanobacteria (isolate C32) isolated from Lake Cernek was used in this study. The biomass obtained by growing this cyanobacteria in BG11 broth was used for the extraction of phycoerythrin. The biomass was extracted by freeze-thaw method in 0.1 M Tris HCl (pH: 8.5). Phycoerythrin was partially purified by ammonium sulfate precipitation (0-20, 20-65). Antioxidant activity and antimicrobial activity on microorganisms of the fractions obtained after DEAE-cellulose ion exchange chromatography were determined using Minimum Inhibition Concentration (MIC) methods. As a result of antimicrobial activity experiments, it was observed that pure phycoerythrin was more effective on Gram (+) microorganisms. Within the scope of the biological activity research of the purified C-FE, ABTS+· cationic radical quenching activity, Fe3+ reducing power, Cu2+ reducing power were investigated. The antioxidant capacity of the purified phycoerythrin was found to be low. This work was supported by the Amasya University FMB-BAP 20-0469 BAP project.