Investigation of the effect of polydatin against sodium fluoride-induced oxidative stress in rats

Fluoride, a highly reactive element, is a halogen element. Fluoride is one of the elements that are important for tooth and bone metabolism and must be taken from outside. However, there is a common misconception that fluorine intake has harmful effects regardless of dose. Polydatin, a glycoside of resveratrol, exhibits a variety of biological functions, including anti-inflammatory and antioxidant. Today, the use of polydatin, which is reported to have cell-protective effects by researchers, as an antioxidant agent against oxidant substances has been a frequently studied subject. In this study, it was aimed to investigate the protective effect of polydatin against sodium fluoride-induced oxidative stress in rats. For this purpose, 600 ppm NaF was given ad libitum to the rats with drinking water. Polydatin was given to the treatment groups at the doses of 25; 50; 100 mg/kg by gastric gavage. In the 14 days study, SOD, MDA, CAT, GSH values from blood, heart, liver, kidney, brain, testis and lung obtained from animals were analyzed. Histopathological examinations of the tissue samples were performed. AST, ALT and ALP levels were measured in serum. In addition, the expression levels of IFN-γ, TNF-α and NFkB inflammatory genes were examined. Sodium fluoride stimulated lipid peroxidation and decreased CAT, SOD and GSH levels in all tissues of rats. It also increased MDA, AST, ALP, and ALT levels. Polydatin application brought SOD, CAT and GSH values closer to control and decreased MDA levels. At the same time, polydatin application brought serum AST, ALT and ALP values closer to the control group. As a result of the study, it was determined that polydatin, a natural antioxidant, had a protective effect on the oxidative stress created by sodium fluoride application. Keywords: Sodium fluoride, polydatin, rat, oxidative stres, lipid peroxidation