Investigation of filling processes of columns used in liquid chromatography

This is the segments of the filling sections of the columns used for phase liquid chromatography (RPLC) technology and their distributions on performance. The main purpose of the research is to reveal the performance criteria such as separation differences, peak symmetry and theoretical plate numbers of column filling change and to present the current calculations comprehensively. Within the scope of the study, column filling pressures produced from C18 material were carried out under constant pressure (500 bar). Methanol and methanol-water mixtures were preferred as filling solvents; the performance of the filled columns was evaluated using quality control tests (theoretical plate count, peak width and peak asymmetry). The new column was compared with the currently used column by optical brightener purity analyses. According to the findings, the performance of the new detail column showed improvement. In particular, an increase in theoretical plates was observed; changes in peak width and peak asymmetry were improved. These results show that the new filling techniques homogenize the problems in the column and increase the column areas. As a result, this study shows that the performance of column filling parameters directly affects the RPLC performance and contributes to new column technologies that provide high efficiency in industrial analyses.