Synthesis of metal-organic frameworks with different morphology for immobilization of tyrosinase enzyme and their use in the removal of phenolic compounds

Tyrosinase enzyme is used to remove phenolic pollutants in wastewater. The aim of this study is to develop environmentally resistant carrier materials immobilised with tyrosinase enzyme to remove phenolic compounds from wastewater. In this context, firstly UIO-66/NH2 was synthesised and incubated with different Cu concentrations. Then tyrosinase enzyme was immobilised on Cu-UIO-66/NH2 carrier. All materials prepared in the study were characterised by different methods. In addition, optimum temperature, optimum pH, storage stability, thermal stability, kinetic parameters, phenol removal of immobilised enzyme were determined and compared with free enzyme. The optimum temperature of the immobilised enzyme was found to be 45 ℃, the optimum temperature of the free enzyme was found to be 40 ℃, and the optimum pH of the free and immobilised enzyme was found to be 7. It was also observed that the immobilised enzyme retained 6% of its initial activity at the eighth use. No significant change was observed in Km and Vmax values of free and immobilised enzyme after immobilisation. The phenolic compound removal of the free enzyme was determined as 70%, while the phenolic compound removal of the immobilised enzyme was determined as 80%. In conclusion, the carrier material prepared in this thesis is a promising material for phenol removal from wastewater.