Molecular characterization of pelemir (Cephalaria syriaca) genotypes collected from the natural flora of turkey and determination of genotyes to be used in breeding

In this study, genetic diversity of 42 genotypes and 2 registered cultivars of pelemir collected from 15 provinces was investigated. 12 SCoT primers were used during the study. A total of 229 bands were obtained and 219 of which were found to be polymorphic, resulting in 91.8% polymorphism. KMaraş1 (G47) and KMaraş3 (G49) genotypes found genetically closest to each other in the SCoT analysis data, while Muş1 (G84) and Mrdn2 (G67) genotypes were the most distant. The lowest PIC value obtained from the primers was obtained from primers SCoT5, SCoT16, SCoT21 and SCoT30 with 0.26, while the highest value was obtained from primers SCoT20 and SCoT29 with 0.32 and the overall mean value was determined as 0.28. In UPGMA cluster analysis, genotypes were divided into 5 main groups. The polymorphism value is normal due to the high diversity rate in natural population areas. There is no previous molecular analysis study on the pelemir plant. This study is a pioneering study on pelemir both in terms of scope and molecular sense. It is thought that it will benefit the breeding studies to be carried out in the future.