Investigation of transition from gametophytic phase to sporophytic phase in some fern species naturally spreaded in Turkey by tissue culture and new generation bioreactor system

This thesis Plantform bioreactor system of temporary immersion system used by solid culture in vitro studies on some fern species naturally grown in Turkey to ensure the transition phase from sporophyte gametophyte stage and plant transformation are provided. Asplenium trichomanes, Asplenium scolopendrium and Asplenium ceterach species were used in the study. In the scope of the thesis, the number of sporophytes in a ½ MS nutrient medium containing combinations of NAA (0, 1.0 mg/L), BA (0, 0.5, 1.0 mg/L) and GA3 (0.5 mg/L) as preliminary work plant height (cm) parameters were examined. As a main study, in vitro development of gametophytes was observed using ½ MS nutrient medium containing combinations of concentrations of NAA (0.1-0.5 mg/L), BA (1.0 mg/L) and KIN (1.0 mg/L). For the Plantform system, 3 hours and 6 hours immersion time was tested in a medium containing ½ MS of Asplenium trichomanes. The best immersion time was determined as 3 hours and this time was applied to the other 2 species. As a result of the study, it was observed that plant conversion rate and quality were better in Plantform system.