Optimization of extraction conditions for phenolics and vitamin C from spindle (Elaeagnus angustifolia L.) using ultrasound-assisted extraction method

In this study, extraction of bioactive compounds such as phenolic compounds, antioxidant substances and ascorbic acid in buckthorn powder was carried out at different rates of ultrasound amplitude (60-80-100 %), different temperatures (40-50-60 °C) and different times (5-10-15 minutes) was applied and its effect on the extract was examined. When the total phenolic analyzes were evaluated according to the extraction results, the highest value was achieved with 568 mg GAE/L at 60°C with 80% amplitude in 5 minutes. Temperature, which is one of the independent variable parameters, was effective on the total phenolic substance extraction. When the EC50 value, which is the antioxidant activity value, was examined, the highest activity was calculated as 338.90 mg/L as a result of the extraction performed at 50°C at 60% amplitude for 5 minutes. The variation of the time, temperature and amplitude parameters selected as independent variables were calculated to be statistically insignificant on the antioxidant activity value. When the ascorbic acid value was examined, the independent variable parameters were statistically significant and the highest value was calculated as 39.79 mg/100 g in the extraction performed at 60°C at 60% amplitude for 10 minutes. When the color values were examined, it was determined that time, temperature and amplitude were statistically significant.