The production potential of mycelia in the enzymes that wild macrofungi possess

In this research, 20 types of macro fungi that are found in our country (Postia stiptica, Pleurotus ostreatus, Meripilus giganteus, Inocybe leiocephala, Stropharia inuncta, Armillaria mellea, Pleurotus sajor-caju, Lentinula edodes, Paxillus involutus, Pleurotus eryngii, Russula fellea, Inocybe flocculosa var. crocifolia, Ganoderma lucidum, Fomes fomentarius, Stropharia inuncta, Tricholoma caligatum, Pleurotus djamor) have been examined for the micelle they contain and the effect on active potential for enzymes. The research was to observe the potential production of the enzymes, protease and amylase, that the macro fungi contain. The conclusions are divided into two outcomes. We put the enzymes into both agar and broth culture and observed their reaction. In conclusion, for amylase, 5 macro fungi came up positive. For protease, there was 10 positive macro fungi. The fungus with the highest amount of amylase was Armillaria mellea with a production of 22.50 U/ml. The fungus with the highest amount of protease was Meripilus giganteus, with a production of 37.69 U/ml. The final part of our research is to figure out the optimum parameter for improving the enzyme production in organisms. To do this, we put organisms in agarose that contain carbon source to measure the production of micelle. There was a control group and experimental group. The control groups had constant factors that favored the production. The experimental group had different pH levels and temperatures to observe how it was affected. In conclusion, the micelles of Armillaria mellea grew in the optimal environment where there was sucrose in the culture, kept at 250C, and had a pH of 6.0. On the other hand, Meripilus giganteus grew colonies at the optimum environment in a fructose culture at 250C with pH of 5.5.