Effect of additional zero valent iron nanoparticle on biohydrogen production from refectory waste

In this thesis, it was aimed to increase biogas and biohydrogen production by adding zero-valent iron nanoparticles (Fe0NP) to refectory wastes by dark fermentation method. Loading was done at 0, 2, 4, 8 and 10 gVS/gVS ratios to determine the highest S/I ratio. The best yield obtained was S/I 4 gVS/gVS reactor. The highest biogas and biohydrogen production occurred at 100 mg/L Fe0NP concentration.The highest TS and VS removal efficiencies were seen in the S/I=4 gVS/gVS reactor. The highest TS and VS removal were determined as 50% and 37%, respectively, in the reactor with 100 mg/L Fe0NP concentration added.The highest total VFA concentration was observed at S/I=4gVS/gVS loading ratio and, 100 mg/L Fe0NP concentration added.In terms of biogas and biohydrogen production, biofilm samples were taken from the S/I=4 gVS/gVS reactor with the highest efficiency and the best results in the addition of Fe0NP, and as a result of examining their microbial distribution, Lactobacillus, Pseudomonas and Clostridium were determined as the most dominant species. Keywords: Zerovalent iron nanoparticle, Dark fermentation, Conductive material, biohydrogen production, biogas production