The effect of curcumin on TRPM2 channel protein in high-fat diet obesity animal model

Aim: In this study, it was aimed to investigate the effect of Curcumin on TRPM2 channel protein in terms of molecular, biochemical and histopathological aspects in experimental obesity model induced by high-fat diet. Material and method: Rats fed with high-fat diet and standard diet for 12 weeks were divided into five groups as control, vehicle, Cur (60 mg/kg bw), YY and YY + Cur. Blood glucose levels, hemodynamic analyses, body and organ weight measurements were taken from animals, and insulin, CRP, TNFα, IL-6, IL-1β and TRPM2 protein amounts were measured from serum and tissue samples by ELISA method. MDA, GSH levels and SOD, CAT activities and TRPM2 gene expression were measured from liver tissues of rats. In addition, liver, heart, vessel, kidney, muscle and white adipose tissue samples were studied histopathologically. Results: Blood sugar was higher in the YY group compared to the other groups (P < 0.005), while YY group showed lower blood pressure and more ECG abnormalities. In the liver, MDA, IL-1β, TRPM2 levels were significantly higher in the YY group (P < 0.005), while SOD activity was lower (P < 0.005); this difference was not observed in the CAT activity, IL-6, TRPM2 gene levels (P > 0.005). Histopathologically, steatosis was found only in the liver of HFD feeding groups; no pathological differences were observed in kidney, heart, vascular, muscle and white fat tissue. Conclusion: The increased blood glucose levels, ALP and cholesterol with the HFD, and abnormal cardiac measurements may cause deterioration in insulin metabolism and cardiovascular problems due to the fatty diet. The increased levels of MDA, IL-1β and TRPM2 in the liver may indicate that oxidative stress will increase with HFD. The decrease in the elevated values with Curcumin may contribute to oxidative stress, lipid metabolism and inflammation.