Synthesis and evaluation of cytotoxic effects of 1-aryl-2-(4-methylpyrazol-1-yl) ethanone oxime derivatives

Aim: Extensive research is being conducted to develop effective anticancer compounds. Azole compounds, in particular, have been utilized in chemical studies focused on cancer treatment. Among azole functional groups, the pyrazole ring structure is the most frequently preferred scaffold. The goal in new therapeutic options is to discover drugs that are less toxic, have reduced side effects, and offer increased patient applicability. This thesis aims to design, synthesize, and investigate the cytotoxic effects of compounds with the "1-(4-chlorophenyl)-2-(4-methylpyrazol-1-yl)ethanone oxime" structure. Material and method: In this study, 11 new compounds were designed, synthesized, and subjected to cytotoxic evaluation. The structures of the compounds were elucidated using data obtained from 1H-NMR, 13C-NMR, and mass spectrometry. Their in vitro anticancer activities against MCF-7, C6, and SH-SY5Y cell lines were also investigated. Results: As a result of the anticancer activity studies, the percentage viability and IC50 values of the synthesized compounds in MCF-7, C6, and SH-SY5Y cell lines were calculated. The results were evaluated based on structure-activity relationships in the C6 cell line, which showed the best activity. Conclusıon: Using these equations, 11 new compounds were synthesized. Compound G10 was found to be effective against the C6 cell line and also exhibited high selectivity. Compounds G8 and G9 showed the lowest activity. The cell viability of the synthesized compounds was evaluated against the SH-SY5Y cell line. Studies proved that compound G10 is less toxic to living cells compared to the other compounds. Keywords: Anticancer, Oxime, Pyrazole