Effects of 1800 MHz magnetic field on colon microflora and pathology in rats and determination of certain gene mutation in colon

The aim of this study was to determine effects of 1800 MHz magnetic field on the amout of colonic Bacteroides and Fusobacterium and colon histopathology in rats and the determination of kirsten rat sarcoma (KRAS) and p53 gene mutations in colon. Three groups were prepared in this study as control, sham and electromagnetic field (EMF) applied group. 1800 MHz RF (radiofrequency) radiation is produced by using an electromagnetic energy generator. The EMF group rats were exposed to electromagnetic field for 12 weeks as 45 minutes per day. No any difference was observed histopathologically between control and sham groups. Mucus epithelial erosions and partial losses were observed in EMF group. The corrupted gland structure, the mucosal edema and the inflammatory cell infiltration were observed. The amout of collagen was increased and fibrosis detected in EMF group. Goblet cell number decreased statistically significant when compared to control and sham groups (P<0.05). The amount of Fusobacterium increased significantly in EMF group compared to controls. The difference was not detected between groups in the amount of Bacteroides. All the samples analysed for KRAS and Tp53 mutations in the colon tissue were found to be wild type. No significant difference was observed between the control group and the EMF applied group. The results indicated that, for 12 weeks 45 min/day exposure to 1800 MHz EMF caused histopatological damage in rat colon. Also, the amount of Fusobacterium is increased. Whereas EMF exposure did not caused to KRAS and p53 mutations in colon tissue. This thesis is supported by No.2014-81 Scientific Research Project (BAP) of Dumlupınar University. Keywords: 1800 MHz, Electromagnetic Field, Bacteroides, Fusobacterium, Colon, KRAS, Rat, Tp53.