Assesment of effective concentration of new herbal anesthetics in juvenile trout (Oncorhynchs mykiss)

Assesment Of Effective Concentration Of New Herbal Anesthetics In Juvenile Trout (Oncorhynchs mykiss) Synthetic anesthetics such as 2-phenoxyethanol, MS-222 (tricaine) and benzocaine are widely used in aquaculture. MS-222 is the only anesthetic agent legally permitted for use in edible fish in the United States and England. The time of expulsion of MS-222 from the fish body by the Drug and Food Organization (FDA) is 21 days. This causes a delay in marketing of fish. The use of different oils obtained from herbal products, which are considered as a good alternative option against synthetic anesthetics, is increasing day by day and their work continues. In studies conducted, it is seen that many studies have been carried out on the anesthetic effects of clove oil. However, there have been few studies on the anesthetic effect of eucalyptus and balm oils. Clove oil in the study; 25, 37.5, 50 µL/L, eucalyptus oil; 300, 350, 400 µL/L and balm oil; 200, 300, 400 µL/L were used in three different concentrations and appropriate anesthetic concentrations were determined in rainbow trout juvenile individuals. Three different trials were made for each dose, and the most appropriate doses were determined. It has been found suitable for rainbow trout with its concentrations of 37,5 µL/L in clove oil, 400 µL/L in eucalyptus oil, and 400 µL/L in balm oil. Keywords: Cloveoil, Eucalyptusoil, Balmoil, Rainbowtrout (Oncorhynchus mykiss), Anestheticagent