Determination and research of lichenese enzyme production, characterization and biotechnological availability of anaerobic fungues

Rumen fungi are microorganisms belonging to the eukaryote domain group that spread in the digestive tract of ruminant creatures. These microorganisms have lignocellulolytic-fibrolotic enzyme groups. Although these enzymes are members of the carbohydrate active enzyme (CAZyme) family, they can also serve as alternative enzyme sources in industrial and biotechnological fields. In addition, it is known that these microorganisms are cultured in anaerobic in vitro conditions and are still very important in the efforts to create a culture collection of related organisms, thanks to the ongoing isolation studies. Therefore, in our study, rumen fungus (Piromyces sp.) was isolated from stool samples of goats distributed in rural areas of Tunceli province and the lichenase enzyme of the related fungus was characterized in vitro. It was determined that the optimum temperature value at which the lichenase enzyme secreted by the Piromyces sp., which is given the MUBAM 4 culture collection number, in which 50℃ and the pH value is 7.0. It was observed that the highest specific activity of the enzyme at the optimum operating temperature was 310.1 μmol/min/mg protein. The highest specific enzyme activity was found to be 100.4 μmol/min/mg protein at the optimum pH value. It has also been determined that the thermal stability of the enzyme suffers a relative activity loss of 10% at the optimum operating temperature. As a result, it was stated that the enzyme has a suitable temperature and pH value for use in biotechnology or industrial areas, but the decrease in thermal stability is important. Keywords: Anaerobic fungus, Piromyces sp., Lichenase, Biotechnology