The determination of genetic polymorphism in Antalya region hair goat by RAPD-PCR

ABSTRACT THE DETERMINATION OF GENETIC POL YMORPfflSM IN ANTALYA REGION HAIR GOAT BY RAPD-PCR Emine ŞAHİN M.S. in the Department of Animal Science Adviser: Assist. Prof. Dr. M. Soner BALCIO?LU Nisan 2005, 48 page The main purpose of this research is to determine genetic polymorphism among Hair goats in Antalya province by RAPD-PCR. RAPD-PCR was employed by using total 20 different primers for 7 male and 7 female hair goats from Antalya province. All the RAPD fragments determined were 100-600 bp in size. The RAPD-PCR using primer Opp08 resulted in 121 DNA fragments. On the other hand, the RAPD-PCR using primer Opp03 resulted in 29 DNA fragments. Total 1363 DNA bands were evaluated. One hundred fourty two bands of the total one hundred fifty three bands were polymorphic. Genetic similarity (Band Sharing Frequency, Fxy ) and genetic distance were 0,6464 and 0,3536, respectively. The expected heterozygosity was estimated as 0,3691+0,1472. KEY WORDS: Hair goat, polymorphism, RAPD-PCR COMMITTEE: Assist. Prof. Dr. M. Soner BALCIO?LU Prof. Dr. İ. Zafer ARIK Assoc. Prof. Dr. Hüseyin BASIM