Investigation of the use possibilities of Aronia melanocarpa asa natural antimicrobial additive in cream additives and coating materials

Diseases caused by pathogenic microorganisms and their development of resistance to antibiotics threaten human health. Plants have been widely used to treat various diseases since ancient times. Aronia melanocarpa (Aronya) is a fruit that has gained popularity recently with its high nutritional value. The aim of this thesis study is to determine the potential of using water extract obtained from Aronya fruits as a natural additive in cream additives and coating materials. Firstly, the antimicrobial activity of the extract on food, clinical, fish-borne pathogens and probiotic candidate lactic acid bacteria (LAB) isolated from breast milk was determined. The extract showed good antimicrobial activity on pathogenic microorganisms. The extract, at the tested concentrations (0.5, 1, 2 mg/disc), had no inhibitory activity on Limosilactobacillus fermentum MA-7 and low antimicrobial activity on other LABs. Then, the lethal concentration of the extract (0, 50 and 100 mg/ml) on Candida albicans ATCC 10231 and L. fermentum MA-7 was determined using the macro-dilution method at 0, 24 and 48 hours. At 48 Hours, a 100 mg/ml concentration of the extract had a 58.11% mortality rate relative to the control on C. albicans ATCC 10231, and a 50 mg/ml extract concentration had a 135.37% survival rate for L. fermentum MA-7. In addition, the sun protection factor (SPF) of the extract and commercial cream-extract mixtures (2.5 - 5 - 10 ml) was determined to evaluate their potential use in cream additives. The extract has a SPF value of 25.77 and the cream-extract mixture at 10 ml concentration has a SPF value of 13. Finally, the antimicrobial activity of the antimicrobial cream and edible film/coating material developed with Aronya water extract and/or L. fermentum MA-7 was determined and potential uses in the pharmaceutical, cosmetic and food industries were determined. The synergistic effect of the extract and L. fermentum MA-7 has high antimicrobial activity on pathogenic microorganisms. According to the results obtained in the thesis study, it has been determined that Aronya fruit can be a natural alternative to many synthetic additives and products in various industries.