In vitro and in silico investigation of antidiabetic effects of some new chalcone and pyrazoline derivative compounds

In this study, the inhibitory effects of synthesized chalcone (K1, K2, and K3) and pyrazoline (P1, P2, and P3) derivatives on α-amylase and α-glucosidase enzymes were investigated using both in vitro and in silico methods. The aim was to contribute to the development of new potential antidiabetic agents. Statistically, P2 molecule was found to have the same IC50 value as acarbose, the standard inhibitor, for α-amylase. The molecule inhibited α-amylase non-competitively with a Ki value of 5.34 µM. All of the pyrazoline molecules studied were found to inhibit α-glucosidase more effectively than acarbose. Among these, P2 molecule with the lowest IC50 value was found to inhibit α-glucosidase non-competitively with a Ki value of 1.14 µM. P2 molecule showed promising results for both α-amylase and α-glucosidase inhibition, indicating a high potential as an antidiabetic agent.