Production of some seconder metabolits from native plant genetic resources in tissue cultures by bioreactors

Within the scope of this thesis, it was aimed to increase the production of secondary metabolites by the application of TIS bioreactor and jasmonic acid in the callus of Salvia officinalis L. following the formation of callus culture in the TIS bioreactor in Rosmarinus officinalis L. and Salvia officinalis L.. For this purpose, preliminary experiments were carried out on media with different plant growth regulator concentrations. In the preliminary trials, the system was optimized with the medium formulation in which the most suitable callus formation was obtained for the TIS bioreactor system. Following preliminary experiments and optimization, callus culture was established the solid culture of Salvia officinalis L. from explants. In order to increase the secondary metabolite content, the obtained calluses were cultured in a solid medium and TIS bioreactor with jasmonic acid, and in a TIS bioreactor without jasmonic acid. At the end of the culture period, the amounts of total phenolic, flavonoid and antioxidants of the extracts obtained from the calluses were determined and the relevant data were compared. According to the data obtained, it was determined that the TIS bioreactor method and jasmonic acid applications increased the secondary metabolite content compared to classical in vitro culture methods and could be used for secondary metabolite production under in vitro culture conditions