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Histological and transmission electron microscopic examination of Chinese goose (Anser cygnoides) liver

2023
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Advisor: Dr. Öğr. Üyesi Hatice Yaren Kuloğlu

Abstract (EN)

The liver, which acts as the main organ in the continuity of energy supply, is the largest gland in the body. The liver, which has functions such as cleansing the body from toxins, synthesizing bile and secreting the synthesized bile, regulating metabolism; It functions as endocrine and exocrine glands. The liver is a complex organ that processes the digested and absorbed substances, stores them for the benefit of other organs, or transfers them to the circulatory system, thus creating a transition zone between the digestive system and the circulatory system. The aim of this study is to examine and interpret the liver of the Chinese goose (Anser cygnoides) histologically. This study was carried out using Chinese geese (Anser cygnoides) taken from a private farm in Aksaray. In the study, 12 Chinese goose (Anser cygnoides), 6 females and 6 males, were used. For premedication, 10-15 mg/kg xylazine was injected intramuscularly into Chinese geese (Anser cygnoides), and 30-50 mg/kg ketamine was injected intramuscularly for anaesthesia. Tissue samples were then taken from the liver. Tissue samples taken were fixed in tubes containing 10% buffered formaldehyde for 1 day and then taken to the washing process. Tissue samples taken were subjected to routine histological tissue follow-up and blocked using paraffin. Tissues blocked using paraffin were divided into 7-8 microns and examined under a light microscope by applying the Hematoxylin Eosin (HE) staining method. Tissues taken for examination under transmission electron microscope were divided into 1 mm pieces, kept in fixation solution consisting of 20 ml of PBS and 3 ml of Glutaraldehyde overnight and washed 3 times with PBS for 15 minutes and then examined in a HITACHI HT 7800 transmission electron microscope. It was observed that the liver of the Chinese goose (Anser cygnoides) did not have the typical lobule structure, consisted of many lobules, and the interlobular septum was thin. It was determined that hepatocytes were oval and centrally positioned and combined to form remark cords, and it was observed that these remark cords were separated by sinusoids. Kupffer cells were found. Lymph nodes were intensely observed in the examined tissues. Bile duct, arteria hepatica and vena porta forming the Kiernan space were encountered. It was determined that each unit with the vena centralis was similar to the lobule structure.

Author

Dr. Ali Burak Çıraklı

How to Cite

Ali Burak Çıraklı (Master Thesis). Histological and transmission electron microscopic examination of Chinese goose (Anser cygnoides) liver, 2023, Aksaray University.

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