The effect of competition on serum apelin, brain fatty acid binding protei̇n levels and some cardi̇ac, stress markers and bi̇ochemical parameters in javelin horses

Javelin is an equestrian team sport played on a horse, which consists of an equestrian athlete throwing his javelin correctly at his opponent, mastering himself and his mount during a battle and thus defeating his opponent, called a game of valor, courage and an indicator of brave. Apelin is a marker of metabolic diseases due to its role in regulating glucose and energy metabolism; FABP7, expressed from astrocytes, lipid metabolism in the brain; cortisol, stress; GRP78, stress level of the endoplasmic reticulum; cTnI are markers used to assess myocardial damage. İn this study, horses that participated in the competition of competition in the javelin (resting state (P0), competition (P1) and the end of the competition (P2)) in the chapel of serum, FABP7, cTnI levels of cortisol, the effect on the levels of some biochemical and GRP78 (glucose, lactate, CK, CK-MB, urea, creatin, AST, ALT, ALP, GGT, LDH, total cholesterol (TC), Triglyceride (TG), VLDL, LDL, HDL) is the study of the relationship parameters. İn this context, a total of 14 Arabian stallions aged between 4 and 10 were used in the study.Blood samples were taken from the vena jugularis of horses in the P0, P1 and P2 periods of the study. The levels of apelin, FABP7, cTnI, cortisol, GRP78 in serum samples obtained from blood samples taken were examined quantitatively by ELISA method, biochemical parameters by spectrophotometric method on Cobas 8000 autoanalyzer.Serum samples were stored at -80° C until the analysis took place. The data of the study were used in IBM SPSS Statistics program to determine the ratio between repeated measurements using non-parametric kruskal wallis H test (cTnI, GRP78, cortisol, glucose, lactate, Dec, urea, creatinine, AST, ALP, LDH).The normal distribution parameters were analyzed by One-Way ANOVA test (apelin, FABP7, CK-MB, ALT, GGT, TK, TG, LDL, VLDL, HDL).When the study groups were examined, the average serum FABP7, GRP78, cTnI, CK, CK-MB, glucose, AST, ALT, ALP values of the P2 periods in the C1 and C2 competitions of the horses in the GA and GB groups were determined according to the values in the P0, P1 periods, While an increase in LDH, urea, creatinine, HDL values was observed, a decrease in mean serum apelin, lactate, TK, TG, LDL, VLDL levels was detected (P<0.05). İn conclusion, the effect of the competition on serum apelin, FABP7, GRP78, Cortisol, cTnI levels and biochemical parameters (glucose, lactate, Na, K, Cl, Ca, Mg, P, Fe, AST, ALT, ALP, LDH, CK, creatinine, urea, TK, TG, HDL, LDL, VLDL) in horses participating in the javelin competition was revealed. Measurement of the level of the hormone apelin,the effect of exercise due to its role in glucose, insulin and energy metabolism; FABP7, lipid metabolism in the brain due to exercise, GRP78, determination of endoplasmic reticulum stress level due to exercise, cTnI, CK, CK-MB, depending on exercise, we think that it may contribute to the evaluation of the cardiovascular system. However, it has been concluded that it is necessary to conduct more comprehensive and molecular-level research in this area, FABP7, apelin exercise, the creation of training and training programs to be applied in horses, their effectiveness and can be useful in the performance management processes of racehorses