Investigation of antioxidant and antibacterial properties of liposomal formulations of Codium sp. extract

In recent years, macroalgae have gained more and more attention for their various health-promoting properties that can reduce the risk of many chronic diseases and even help prolong lifespan. Codium (Bryopsidales) is a diverse genus of green marine macroalgae belonging to the Codiaceae family. The bioactive compounds and polysaccharides found in Codium have different pharmacological effects, including immunostimulating, anti-inflammatory, anticoagulant, antioxidant, anti-obesity, antiviral and anticancer. Liposomes are small artificial vesicles of spherical shape that can be formed from cholesterol and natural, non-toxic phospholipids. Besides being biodegradable and biocompatible, liposomes' ability to release the encapsulated substance at any time while minimizing the toxic effect is one of the most important reasons for their preference. In this thesis, antibacterial and antioxidant properties of both extract and extract liposome formulations of Codium sp. collected from Marmara and Aegean seas were investigated. Extracted by two different methods, Codium sp. Marmara and Aegean samples were exposed to ethanol at 40 °C and room temperature. Codium sp. The disc diffusion method and the minimum inhibitory concentration (MIC) method were used to determine the antibacterial effect of the samples. Following the MIC test, the minimum bactericidal concentration (MBC) was measured. According to the disk diffusion test results, Codium sp. In both samples, it shows antibacterial properties with a diameter of 8 mm. Codium sp. DPPH radical scavenging activity, total phenolic content (TPC) and encapsulation efficiency test were performed on both samples. The solutions prepared from the extracts at concentrations of 100-200-400-800 g/mL enabled the determination of the TPC amount. According to the results, TPC results at each concentration in the two extracts were compared, and the amount of TPC in the Aegean sample was found to be higher than in the Marmara sample. Codium sp. The encapsulation efficiency of Marmara and Aegean extracts was calculated as 67.56% and 76.92%, respectively. Since color change is expected primarily in the DPPH analysis, Codium sp. Color change was investigated in Marmara and Aegean samples. The EC50 values of Marmara and Aegean extracts were calculated as 429.21μg/mL and 834.29 μg/mL, respectively. According to the results of this thesis study; A small amount of extract to be formulated with liposomal formulation is sufficient for antimicrobial effect. In addition, when the liposome formulation and the extract were compared, it was observed that the formulation did not cause any loss in the antioxidant activity of the extract.