Çoklu materyal içerikli doku iskelelerin üretimi ve kontrollü materyal saliminin optimizasyonu

Implantable polymeric hydrogels have been widely investigated for drug delivery systems. Collagen and Alginate are one of the most abundant natural polymers, and their biocompatibility and biodegradability make them a favourable material for implantable hydrogel fabrication. The addition of degradable polymeric microspheres can improve the hydrogel drug release behaviour. Poly caprolactone (PCL) have been studied for the use in drug delivery systems, and they can act as drug delivery vehicles to improve the delivery of different types of therapeutic agents. In this work, Vascular Endothelial Growth Factor (VEGF) and Rapamycin loaded PCL microparticles were fabricated by double emulsion technique, a Col/Alg hydrogels fabricated using 3D printing approach, and. Several tests including scanning electron microscopy, in vitro releasing, were utilized to characterize the microspheres. In addition, the physical properties of hydrogels, including mechanical properties, degradation properties, were investigated. The resulting VEGF-loaded microspheres had average sizes of 900 2000 nm and the in vitro release test confirms the sustained stable releasing profile of VEGF over a time of 15 days. On the other hand, the tensile properties and degradability of hydrogels, were readily tuned by increasing the concentration of Alginate without changing the concentration of collagen. These results imply that these implantable hydrogels are suitable candidates for applications in the fields of drug delivery and organ transplantation.