Investigation of gene expression of carbonic anhydrase isoenzymes in denerved rat in adipose tissue

The sympathetic nervous system plays an important role in the regulation of adipose tissue metabolism. In adipose tissue denervation studies, it was observed that lipolysis in denervated tissue decreased and adipose tissue mass increased. The size increase of fat cells is shaped by excessive TG storage. In this project, it was investigated how the CA II, VA and VB isoenzymes, which are substrate providers in de novo lipid synthesis, change in white adipose tissue due to denervation. In the project, 16 male rats, 180-220 g, 4-6 weeks old, were used and two study groups were formed. Group 1: Right retroperitoneal adipose tissue was completely denervated and sacrificed 1 month later (left retroperitoneal adipose tissue was not denervated and was used as a control). Group 2: Right retroperitoneal adipose tissue was completely denervated and sacrificed 3 months later (left retroperitoneal adipose tissue was not denervated and was used as a control). After sacrification, both retroperitoneal adipose tissues were removed and their weights were weighed. cDNA synthesis and norepinephrine measurement were performed in tissue samples. In group 1, the level of Car2, Car5A and Car5B gene expression in the denervated right retroperitoneal adipose tissue was found to be increased when compared to the undenervated left retroperitoneal adipose tissue. There was no difference in expression levels after three months of denervation. As a result, it was concluded that gene expressions of CA2, CCa5A and CA5B isoenzymes in denervated adipose tissue in the first month following denervation changed in a way to support de novo lipid synthesis and this contributed to the mass increase in denervated adipose tissue.