Effect of anti-TNF-? antibody administration on bacterial translocation in early period after experimental intestinal obstruction

Objective: To investigate the effects of Anti-TNF-? monoclonal antibody (Infliximab) administration on bacterial translocation in an intestinal obstruction model within early period in rats.Material and Method: 50 Wistar-Albino type female rats with weights varying between 199 ? 250g were included in the study. Rats were equally distributed to five groups as following: Control (I. group n= 10), NS 24th hour (II. group n= 10), infliximab 24th hour (III. group n= 10), NS 48th hour (IV. group n= 10) and infliximab 48th hour (V. group n= 10). Intraperitoneal injection of NaCl 0.9% was administered to I., II. and IV. group at the dose of 3 cc. Intraperitoneal injection of infliximab at 10 mg/kg was administered to III. and V. group. Relaparotomy was performed on I., II. and III. group at 24th hour and samples of tissue and blood were acquired. Intraperitoneal injection of NaCl 0.9% at the dose of 3 cc was administered to IV. group a second time at 24th hour and intraperitoneal injection of infliximab at 10 mg/kg was administered to V. group a second time at 24th hour. Relaparotomy was performed on IV. and V. group at 48th hour and samples of tissue and blood were acquired. Having obtained intracardiac blood samples, rats were sacrificed at the end of 24th and 48th hours. Tumor necrosis factor alpha, interleukine-6, glutathione reductase, glutathione, glutathione peroxidase, malondialdehyde, myeloperoxidase, superoxide dysmutase and nitric oxide levels were measured in the samples of blood and tissue acquired. In the microbiological evaluation of tissue samples, samples were stained according to the Gram staining protocol and identified with regard to Gram-staining characteristics, denoted as negative and positive, and to their responses against various enzyme reactions.Results: No difference was found between infliximab received group and control groups at the end of 24th and 48th hours regarding tumor necrosis factor alpha, interleukine-6, glutathione reductase, glutathione, glutathione peroxidase, superoxide dysmutase, malondialdehyde, myeloperoxidase, and nitric oxide values in the tissue and blood (p>0.05). Proliferation in mesenteric lymph nodes was lower in V. group (p<0.05).Conclusion: TNF blockage by infliximab administration reduces the bacterial translocation into mesenteric lymph nodes in the intestinal obstruction model. However, mechanism of action of infliximab administration on microenvironment could not be demonstrated completely. Besides, it may be said that it is a systemic agent, which could support the mucosal barrier in the early period of intestinal obstruction. More detailed studies are required to be performed to reveal the possible new clinical role ofKeywords: Anti-TNF ?? monoclonal antibody, Infliximab, Intestinal obstruction, Bacterial translocation