The investigation of adipose tissue's oxidant-antioxidant balance in dietary-induced obesity

The oxidant-antioxidant balance alterations, associated with obesity, have been previously reported. However, the extend of such changes in the fat tissues located at different regions of the body is not clear. Similarly, the relation between the adipokin release and the oxidant-antioxidant balance remains to be elucidated. In this study, the rat model of high fat diet obesity was used to determine the oxidant-antioxidant balance and the relation to the adipokin release in fat tissues of different body regions. How antioxidant use effects the changes occured also investigated. Control, obese and obese +2 g/L N-acetylcysteine groups were established. Each group consisted 6 Sprague-Dawley rats. Control rats were fed by standart food and obese and N-acetylcysteine group were fed by high fat diet. The serum samples and fat tissue samples from subcutaneous, visceral and perirenal pads were collected. The concentrations of leptin, adiponectin, apelin, resistin, insulin, interleukin-6, tumor necrosis factor alpha, retinol binding protein-4 were determined in the serum samples. In the fat tissue samples, messenger ribonucleic acid levels of leptin, adiponectin, apelin, resistin and nicotinamide adenine dinucleotide phosphate-oxidase were measured by real time polymerase chain reaction. Additionally, the amounts of malondialdehyde, protein carbonyl group, glutathione, superoxide dismutase, catalase and glutathione peroxidase activities were determined. Serum leptin, perirenal malondialdehyde and subcutaneous glutathion levels increased in obese group (p=0.003, p=0.004, p=0.032, respectively) and visceral superoxide dismutase, visceral catalase and perirenal catalase activities decreased in obese group (p=0.009, p=0.009, p=0.002, respectively). Leptin and apelin messenger ribonucleic acid levels increased in obese group (p=0.04, p=0.03), respectively). It was suggested that, there are differences in oxidative stress responses and adipokine expression profile for differently located adipose tissues in rats fed high fat diet.