Effect of boron and F68 pluronic block copolymer combination on adipogenic differentiation of human adipose stem cells

Obesity has become a crucial health problem in public worldwide, thereby making weight loss inevitable to recover for obese as well as overweight people. However, weight loss and weight control drugs provided by the current food industry have failed in the long-term maintenance of weight control. Therefore, it is a challenge in the field to find new types of drugs that could be valuable for the prevention and treatment of obesity. Since obesity is a consequence of the formation of new mature adipocytes from undifferentiated precursors, drugs that might control adipogenesis could be beneficial for the treatment of obesity. Therefore, developing new, safe, and effective complex for adipocyte inhibition has been a desirable field of research. In the current study, sodium pentaborate pentahydrate (NaB), pluronics F68 and their combination were investigated on adipogenic differentiation by using human adipose stem cells (HASC) in vitro. The data showed the suppressed expression of adipogenesis-promoting genes that are peroxisome proliferator-activated receptor-γ (PPARγ), fatty acid binding protein (FABP4) and adiponectin. In this report, we demonstrated that NaB and F68 combination prevented adipocyte differentiation by inhibiting the adipogenic transcriptional program leading to a decrease in lipid accumulation into the cell. Thus, our study using HASCs will be a useful guide for prevention of adipose tissue-associated diseases including obesity and diabetes.