A new alternative technology for ELISA: Reusable affinity wells

Enzyme-linked immunosorbent assays (ELISA) are plate-based assays designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones. Detection is accomplished by assessing the conjugated enzyme activity via incubation with a substrate to produce a measurable product. An enzyme can be linked directly to the primary antibody or a protein such as streptavidin if the primary antibody is biotin labeled. The most commonly used enzymes are horseradish peroxidase (HRP) and alkaline phosphatase (AP). ß-galactosidase, acetylcholinesterase and catalase can be used for performing the ELISA. The choice of substrate depends upon the instrumentation (spectrophotometer, fluorometer) available for signal-detection. ELISA is performed in 96-well (or 384-well) polystyrene plates which will bind antibodies and proteins are disposable. Protein A, G, C coated, Biotin coated, Streptavidin coated, nickel coated, copper coated, amine coated yada antibody coated modified microplates are prefered for ELISA.In this study, the re-usable microplates by the modification of the polystyrene microplate wells used in the ELISA procedure have been obtained. Polystyrene plate wells, coated with the amine by plasma method, have been made suitable for antibodies or other proteins cross-linking to the surface by modification using fotosensitive monomers.