Investigation of antimicrobial activity of i̇njectable platelet-rich fibrin on oral microbial pathogens i̇n vitro

The aim of this study is to investigation of time-dependent change of antimicrobial efficacy of i-PRF on Enterococcus faecalis and Candida Albicans. In this study, the antimicrobial efficacy of i-PRF obtained from blood samples taken from 50 systemically healthy patients who applied to Karadeniz Technical University Faculty of Dentistry Department of Periodontology between August and November 2020 was evaluated. For the study, 500 μl Test 1 and Control 1 group samples were prepared by mixing 50 μl E.faecalis microorganism suspensions with 450 μl e-TZF and 450 μl whole blood separately. Similarly, 500 μl of Test 2 and Control 2 group samples were prepared by mixing 50 μl C.albicans microorganism suspensions with 450 μl e-TZF and 450 μl whole blood separately. The samples were inoculated at the beginning, 30th, 60th, 120th, 180th and 240th minutes and the number of colonies formed after the incubation in the cultivated mediums were examined. In comparison between test and control groups, it was found that the number of colonies formed in the Test 1 and Test 2 groups were significantly lower than the Control 1 and Control 2 groups at all times except the baseline (p<0.001). In-group evaluations, the number of colonies formed in Test 1 group and Test 2 groups samples were found to be significantly lower at other times compared to baseline values (p<0.001). When the time-dependent change of antimicrobial efficacy of i-PRF on E.faecalis and C.albicans was examined, it was found that the antimicrobial activity on E.faecalis was the highest at the 60th minute and the antimicrobial activity on C.albicans at the 30th minute. Although it decreased depending on the time, it was observed that the antimicrobial activity of i-PRF on E.faecalis and C.albicans continued until the 240th minute. As a result of the data obtained from our study, it was found that i-PRF showed antimicrobial activity on E.faecalis and C.albicans. Further clinical and laboratory studies are needed to evaluate the time-dependent effectiveness of e-TZF on different oral pathogens.