Evaluation of Bisphenol-A release from different fissure sealant materials In Vitro

This study aimed to assess the release of bisphenol-A (BPA) from fissure sealant materials within 24 hours, considering different polymerization times and immersion solutions. In this study, 10x2 mm disks were obtained from Fuji Triage, Fissurit FX, and Clinpro™. Each of the three groups comprised 16 disks, totaling 48 disks examined. The fissure sealant materials used in the experimental groups were as follows: Group 1: Clinpro™, Group 2: Fuji Triage, and Group 3: Fissurit FX. Two polymerization times, 20 and 40 seconds, were employed. The groups were divided into four subgroups: immersion in artificial saliva for 20 seconds, artificial saliva for 40 seconds; ethanol for 20 seconds, and ethanol for 40 seconds (n=3, beta=1, power 80). The disks were then stored in artificial saliva and ethanol solutions for 1 day. Statistical analysis was performed using SPSS (Statistical Package for Social Sciences) for Windows version 24.0. There was a highly statistically significant difference among fissure sealants regarding BPA release (p=0.000). BPA release was detected in all fissure sealant materials. The lowest BPA release was observed with Fuji Triage at 40 seconds of polymerization in artificial saliva, while the highest release occurred with Fissurit FX at 20 seconds of polymerization in ethanol. Fissure sealants with a high resin-containing filler ratio exhibited low BPA release. Although BPA release occurs following fissure sealant application, the measured amount of BPA release, even after one day, suggests that pit and fissure sealants can be used safely. However, additional measures should be taken to eliminate the oxygen inhibition layer post-polymerization of fissure sealants or to prevent saliva interaction during application.