Establishment of organoid from the cells of mouse small intestine crypts by 3-d culture

Small intestinal epithelium is one of the most convenient tissue for mammalian adult stem cell studies thanks to its simple structure and rapidly regeneration ability. The intestinal epithelium consists of crypt and villus. Villus is enriched by several contiguous, proliferative 'crypt' compartments within the underlying submucosa. In this study, small intestine is chosen due to its property of rapidly proliferation and differentiation ability of stem cells. Tissue stem cells function with the support of niche (microenvironment) cells. Therefore, in tissue culture studies various cell types should be used rather than a single type of cells. Scientists trend to living organisms when they need this kind of situations. Challenges of studying with living organisms have led to the establishment of organoids which are obtained by three-dimensional cultures. Mini-organ like structures generated by providing necessary conditions for stem cells which are originated from target organs and able to form stroma, are called organoids. Organoid systems allow different cell types originated from same tissue to grow in the same culture dish. In this study it is aim to establish 3-dimensional culture system and organoid. To form the organoid system will provide significant benefits, such as modelling the physiological environment of an organ and long term replication without genetic instability for future studies including crypt biology, stem cell research and marker gene search, development of anti-cancer treatment, etc. In this study, we have been formed organoids from the cells isolated from mice intestine crypt structures using 3-dimensional culture techniques. We strongly suggest that establishment of organoids will contribute to our university, country and the studies which have been shown great progress and attracts growing interest in the world, recently.