Evaluation of the effects of different temperatures on blood cell?s morphology and life time

Introduction: As seen in most of the body cells, Apoptosis is the most common cellular death form in the blood cells. One of the factors that affect apoptosis in blood cells is temperature. Our aim of this study is to analyze apoptotic cell death related with the change of the temperature in vitro, evaluate the role of HSP70 protein during this process and measure the potential effect between the changing heat and the rate of all blood cells and display whether these criteria make differences between the genders.Materials and Methods : In this study, blood samples taken from quite healthy 6 women and 6 men from 18 to 25 years old have been used. These samples were assembled into 7 sub-groups within itself and by increasing the temperature 2 oC per hour, all the samples? heat was raised from 37 oC to 43 oC. During the heating, some part of the sample that was heated to 39 oC was cooled to 37 oC after an hour, this same process was applied on the samples that were heated to 41 oC and 43oC. At the end of the period, 2 smears were taken from the blood samples for Giemsa and Immunocytochemical stainings. From the same blood samples taken with the same method at the each steps of the experiment, complete blood cells counted and, biochemically, HSP70 titer was measured. All the data were evaluated statistically.Results: At the end of the study, it was confirmed that temperature, especially at higher temperature, raised apoptotic cellular death rate in leukocytes. On the other hand, it was seen that in the groups with decreasing temperature ? surprisingly, when cooled to 37 oC from higher temperature rapidly- the death of the cells increased. Besides, there were no significant differences between the levels of HSP70 in all groups. Likewise, despite apoptotic rate was increased in higher temperature, there were no significant increasing in the proportion of blood elements. At last, all of the data got from all criteria revealed that there were no significant differences between women and men.Discussion: All the data revealed that increasing temperature triggers apoptotic cell death in blood leukocytes but this increasing did not affect the number of blood cells clearly. On the other hand, in this model, HSP70 expression is not triggered by changing of temperature.