Micro- and nanoparticles that will be used for pharmaceutical purpose, are attached to antibody and research of their efficiencies

To be able to survive, cancer cells can manage natural and artificial attempts which can cause their extermination. They do so through an incredible variety of pathways that continues to deceive both our immune system and our thought strategies to fight cancer. But this is just the beginning of cancer. Cancer accomplishes all of the above through the use of the most sophisticated weapon, evolution. Current therapies can destroy cancer cells, but not without destroying surrounding tissues. Even if the treatment is successful, resurgences are the norm, and in an even more aggressive form than before. In recent years, enhanced anticancer drugs are out of conventional therapy and they are becoming a targeting therapy. Carier systems are used for drugs that are sent to target area in the body. First utility of this, drugs will be an appropriate interaction in target area and at required speed. Second utility of targeting therapy is decreasing of doses and drugs will be only in the target area. Thus, any side effects would decrased substantially. Monoclonal antibodies (mAb) intended for several target molecules are quite effective tools for cancer therapy. Despite of their high costs, they are in conventional therapy because of their high selectivity and good toxicity profiles. EGFR is one of target molecules. In this study, mAb anti-HER2 that inhibite HER2, is intended to conjugate to micro and nanoparticles that are drug carier systems. Create of carier systems need certain conditions such as treatment with organic solvent, stirring in high speed or ultrasonication, heating or freezing. Effect of these conditions were researched. Liposome and solid lipid nanoparticles were prepared with sonication and antibodies were encapsulated them. As a result, a drug carier system was revealed without needing a chemical reaction or pegilation. Obtained particles were found as nano-sized, showing homogeneous distribution, good surface charge for transfection ability and having low cytotoxic effects. Significant effects were not determined when the applied stress conditions of formulation on the antibody.