Determination of favipiravir by chromatographic method in pharmaceutical preparations

In this study, a method was developed and validated by using high performance liquid chromatography method for determination of favipiravir and its impurities in pharmaceutical preparaitions. Chromatographic seperations were carried out on the C18 column using 10 mM potassium dihydrogen phosphate pH 4.0 : acetonitrile as mobile phase A and acetonitrile as mobile phase B. Mobile phase flow rate was 1.5 mL/min. PDA detector was used at 238 nm. Linear range was 0.5 – 3 μg/mL. Detection and quantitation limits were found 0.07 μg/mL and 0.2 μg/mL, respectively. Accuracy studies were carried out within 50% and 150% of the limit value. Validated method is fast, precise and reproducible. This method can be used determination of favipiravir impurities for film coated tablets. Keywords: Favipiravir, Impurity, HPLC, Validation, Pharmaceutical Preparation