Investigation of harmful effects of formaldehyde on the foetal morphological structure, liver development which applied to pregnant rats; the possible protective effects of chrysin against these damage

Aim: We aimed in this experimental study to investigate for potentially harmful effectsof formaldehyde exposure during pregnancy on the development of fetuses and livermorphology and to determine possible protective effect of chrysin to the harmful effects.Material and Methods: 67 adult Wistar Albino genus male rats whose weights arebetween 200-250 gram were used in this study. 67 female rats were divided into 7groups and attempted to create pregnancy. 12 non-pregnant rats were excluded from thestudy. We injected physiological saline to group I intraperitoneally (i.p.), 20 mg/kgchrysin (CH) to group II by gavage, 0,1 mg/kg formaldehyde (FA) to group III i.p., 1mg/kg FA to group IV i.p., 0,1 mg/kg FA i.p. and 20 mg/kg CH by gavage to group V,1 mg/kg FA i.p. and 20 mg/kg CH by gavage to group VI and corn oil to group VII bygavage between 7th and 20th day of pregnancy every other day. Fetuses were removed bycesarean section on 20th day of pregnancy. Morphological parameters (fetal weight andfetal liver weight) of fetuses examined. Histological staining and biochemical analyzeswere performed to determine the development of the liver are affected or not.Findings: Statistically significant decrease was found in liver weight and fetal weight inthe group treated with FA. Statistically significant decrease was found onmegakaryocyte number in the group treated with FA 0,1 and FA 1. Histopathologicalchanges were observed in the group treated with FA 1. FA biochemically reduced thelevel of glutathione (GSH) and augmented the level of malondialdehyde(MDA).Results: Exposure to low doses of formaldehyde during pregnancy causing oxidativestress in the fetus and causes morphological, histological and biochemical negativeeffects. Chrysin showed antioxidant effects on morphological and biochemicalparameters but inadequate to correct histological changes.viiKey words: Formaldehyde, Chrysin, Rat fetuses, Morphological structure, Developmentof liver.Aim: We aimed in this experimental study to investigate for potentially harmful effects of formaldehyde exposure during pregnancy on the development of fetuses and liver morphology and to determine possible protective effect of chrysin to the harmful effects.Material and Methods: 67 adult Wistar Albino genus male rats whose weights are between 200-250 gram were used in this study. 67 female rats were divided into 7 groups and attempted to create pregnancy. 12 non-pregnant rats were excluded from the study. We injected physiological saline to group I intraperitoneally (i.p.), 20 mg/kg chrysin (CH) to group II by gavage, 0,1 mg/kg formaldehyde (FA) to group III i.p., 1 mg/kg FA to group IV i.p., 0,1 mg/kg FA i.p. and 20 mg/kg CH by gavage to group V, 1 mg/kg FA i.p. and 20 mg/kg CH by gavage to group VI and corn oil to group VII by gavage between 7th and 20th day of pregnancy every other day. Fetuses were removed by cesarean section on 20th day of pregnancy. Morphological parameters (fetal weight and fetal liver weight) of fetuses examined. Histological staining and biochemical analyzes were performed to determine the development of the liver are affected or not.Findings: Statistically significant decrease was found in liver weight and fetal weight in the group treated with FA. Statistically significant decrease was found on megakaryocyte number in the group treated with FA 0,1 and FA 1. Histopathological changes were observed in the group treated with FA 1. FA biochemically reduced the level of glutathione (GSH) and augmented the level of malondialdehyde(MDA).Results: Exposure to low doses of formaldehyde during pregnancy causing oxidative stress in the fetus and causes morphological, histological and biochemical negative effects. Chrysin showed antioxidant effects on morphological and biochemical parameters but inadequate to correct histological changes.