Comparison of oxidative stress biomarkers in gcf, saliva and blood serum between healthy pregnants and pregnants with gestational diabetes

The present work was supported by the Research Fund of Istanbul University. Project No. 3367 Key words: periodontal diseases, gingivitis, periodontitis, gestational diabetes, oxidative stress Aim: The aim of this study is to comparatively evaluate oxidative stress biomarkers in saliva, Gingival Crevicular Fluid (GCF) and, serum between healthy pregnants and pregnants with gestational diabetes (GDM). The links between periodontal health/disease, oxidative stress and gestational diabetes had been investigated. Due to the potential of oxidative stress to activate common pro-inflammatory ways of diabetes mellitus (DM) and periodontitis, it is pointed out that oxidative stress as a common etiopathogenic factor for both diseases. Materials and Methods: The study group consisted of 80 healthy pregnants and 80 pregnants with GDM. After medical and dental anamnesis, plaque index (PI), gingival index (GI), bleeding on probing (BOP), probing depth (PD) and clinical attachment level (CAL) measurements were performed. GCF, saliva and blood serum were collected. Total Oxidant Status (TOS) and Total Antioxidant Status (TAS) markers in GCF, saliva and serum were analyzed to evaluate local and systemic oxidant-antioxidant mechanisms. Results: PI, GI, BoP, PD and CAL, bruxism, smoking status, Stage I and Stage II periodontitis were significantly higher in GDM group compared to the control group. Serum TAS / TOS and saliva were significantly lower in the GDM group than in the TAS / TOS control group (p = 0.0001). This may be due to the high damage caused by TOS in the GDM group or the consumption of all antioxidant enzyme sources to compensate for this damage by TAS. The mean GFC TAS U / ml and GFC TAS / TOS of the GDM group were statistically significantly lower than the control group, and the GFC TOS U / ml was significantly higher than the control group (p = 0.0001). In the GDM group, we can say that both the high oxidant activity and the antioxidant sources are consumed to a large extent in order to balance this high oxidant level in GFC.